The Mouse CD8a Monoclonal Antibody (APC-conjugated) is a vital tool for researchers studying immune responses and cell-mediated immunity in a mouse model system. This antibody, known as 53-7.6 (AGEL1338), specifically targets CD8a, a cell surface glycoprotein found on cytotoxic T cells. By binding to CD8a, this monoclonal antibody enables the detection and analysis of CD8a-expressing cells in various tissues and experimental setups.CD8a, also referred to as the T-cell co-receptor, plays a crucial role in antigen recognition and activation of cytotoxic T cells, which are essential for immune surveillance and defense against infected or abnormal cells. The presence and function of CD8a-expressing T cells are central to adaptive immune responses, making the Mouse CD8a Monoclonal Antibody a valuable asset in immunology research, vaccine development, and studies on infectious diseases and cancer immunotherapy.
This APC-conjugated antibody offers the advantage of fluorescence detection, allowing for flow cytometry and other fluorescent-based assays to identify and quantify CD8a-positive cells with high specificity and sensitivity. Whether investigating T-cell activation, immune cell populations, or immune responses in disease models, the Mouse CD8a Monoclonal Antibody (APC-conjugated) 53-7.6 (AGEL1338) provides a reliable tool for precise and reproducible analysis in preclinical and basic research settings.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
P01731
Gene ID:
12525
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
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