Mouse Chromodomain Helicase DNA Binding Protein 4 (CHD4) ELISA Kit
The Mouse CHD4 (Chromodomain Helicase DNA Binding Protein 4) ELISA Kit is specifically designed for the accurate measurement of CHD4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.CHD4 is a critical protein involved in chromatin remodeling, gene regulation, and DNA repair processes. It plays a crucial role in various biological pathways, including embryonic development, cancer progression, and immune responses.
Detecting and quantifying CHD4 levels can provide valuable insights into these pathways, making it a valuable tool for researchers studying gene expression, epigenetics, and cellular processes.Overall, the Mouse CHD4 ELISA Kit from Assay Genie is an essential tool for research in molecular biology, genetics, and cancer biology, providing accurate and reliable measurements of CHD4 levels in mouse samples.
Product Name:
Mouse Chromodomain Helicase DNA Binding Protein 4 (CHD4) ELISA Kit
Product Code:
MODL00260
Size:
96 Assays
Target:
CHD4
Synonyms:
Mi-2b, Mi2-BETA, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
