The Mouse CK-MB (Creatine Kinase-MB) Isoenzyme CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CK-MB levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications.Creatine Kinase-MB is an important enzyme found in heart muscle cells and is released into the bloodstream following a heart attack or other cardiac events.
Monitoring CK-MB levels can provide valuable information on heart health and function, making this kit a valuable tool for studying cardiac diseases and developing potential therapies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CK-MB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CK-MB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CK-MB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CK-MB. You can calculate the concentration of Mouse CK-MB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
89-103
96-108
Average (%)
96
96
102
1:4
Range (%)
93-108
100-116
96-110
Average (%)
99
107
101
1:8
Range (%)
103-118
99-114
98-110
Average (%)
110
106
104
1:16
Range (%)
99-117
100-114
93-105
Average (%)
108
106
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
101
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.33
93.3
338.33
42.44
92.99
358.57
Standard deviation
3.74
7.65
28.25
5.27
8.81
24.56
C V (%)
8.07
8.2
8.35
12.42
9.47
6.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CK-MB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CK-MB in samples. No significant cross-reactivity or interference between Mouse CK-MB and analogues was observed.
