The Mouse COL10 (Collagen Type X) CLIA Kit is specifically designed for the quantification of Collagen Type X levels in mouse serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides accurate and reliable results for various research applications.Collagen Type X is a key component of the extracellular matrix, playing a crucial role in bone development and growth plate formation.
Abnormal levels of Collagen Type X have been linked to skeletal disorders and cartilage degeneration, making it a valuable biomarker in the study of musculoskeletal diseases and potential therapeutic interventions.Overall, the Mouse COL10 (Collagen Type X) CLIA Kit is an essential tool for researchers looking to investigate the role of Collagen Type X in skeletal development and disease pathology.
Product Name:
Mouse COL10 (Collagen Type X) CLIA Kit
SKU:
MOES00192
Target:
Mouse COL10 (Collagen Type X)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL10. You can calculate the concentration of Mouse COL10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
95-112
90-104
Average (%)
93
103
96
1:4
Range (%)
89-102
92-105
90-103
Average (%)
95
98
98
1:8
Range (%)
95-106
97-108
92-105
Average (%)
100
102
98
1:16
Range (%)
98-111
103-115
95-111
Average (%)
104
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
98-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.81
568.35
1620.57
194.64
531.3
1642.67
Standard deviation
19.24
54.96
129.81
24.02
39.95
111.87
C V (%)
10.41
9.67
8.01
12.34
7.52
6.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse COL10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL10 in samples. No significant cross-reactivity or interference between Mouse COL10 and analogues was observed.
