The Mouse Col2 (Collagen Type 2) CLIA Kit is specifically designed for the quantitative determination of collagen type 2 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Collagen type 2 is a key component of cartilage and plays a critical role in maintaining the structural integrity of joints. Changes in collagen type 2 levels have been linked to conditions such as osteoarthritis and rheumatoid arthritis, making it a valuable marker for studying these diseases and developing potential therapeutic interventions.
With the Mouse Col2 (Collagen Type 2) CLIA Kit, researchers can confidently measure collagen type 2 levels in mouse samples, providing valuable insights into the pathogenesis of joint-related disorders and facilitating the development of novel treatment strategies.
Product Name:
Mouse COL2 (Collagen Type 2) CLIA Kit
SKU:
MOES00210
Target:
Mouse COL2 (Collagen Type 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL2. You can calculate the concentration of Mouse COL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
97-113
90-107
Average (%)
108
103
98
1:4
Range (%)
91-102
101-116
97-114
Average (%)
96
109
105
1:8
Range (%)
97-112
99-114
99-113
Average (%)
104
105
105
1:16
Range (%)
94-110
100-113
95-108
Average (%)
101
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
106
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
95-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.4
145.13
421.24
47.29
135.47
401.15
Standard deviation
5.66
16.4
36.77
4.74
10.66
44.09
C V (%)
11.23
11.3
8.73
10.02
7.87
10.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse COL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COL2 in samples. No significant cross-reactivity or interference between Mouse COL2 and analogues was observed.
