Mouse Collagen Type III Alpha 1 (COL3a1) ELISA Kit
The Mouse Collagen Type III Alpha 1 (COL3A1) ELISA Kit from Assay Genie is specifically designed for the accurate detection of mouse collagen type III alpha 1 levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it suitable for a wide range of research applications.Collagen type III alpha 1 is a critical component of extracellular matrix in various tissues, including skin, blood vessels, and organs. Abnormal levels of this protein can be indicative of fibrotic disorders, connective tissue diseases, and other pathological conditions.
Therefore, the Mouse Collagen Type III Alpha 1 (COL3A1) ELISA Kit is an essential tool for studying these diseases and evaluating potential therapeutic interventions.Trust Assay Genie's Mouse Collagen Type III Alpha 1 (COL3A1) ELISA Kit for reliable and accurate measurements of mouse collagen type III alpha 1 levels in biological samples, facilitating groundbreaking research in the field of collagen-related diseases and potential treatment strategies.
Product Name:
Mouse Collagen Type III Alpha 1 (COL3a1) ELISA Kit
Product Code:
MODL00290
Size:
96 Assays
Target:
COL3a1
Synonyms:
COL3-A1, COL3A1, EDS4A, Collagen Type III Alpha 1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Collagen Alpha-1(III)chain
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
