The Mouse COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit is specifically designed for the accurate detection of COMP levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing dependable and consistent results, making it perfect for various research applications.COMP, also known as thrombospondin-5, is a key protein found in cartilage and other connective tissues. It plays a critical role in maintaining the structural integrity of cartilage and is implicated in conditions such as osteoarthritis and rheumatoid arthritis.
As such, COMP serves as a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Mouse COMP ELISA Kit provides researchers with a reliable tool for quantifying COMP levels in mouse samples, facilitating a deeper understanding of cartilage biology and related pathologies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COMP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COMP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COMP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COMP. You can calculate the concentration of Mouse COMP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
90-102
98-110
Average (%)
90
95
104
1:4
Range (%)
93-109
85-100
85-97
Average (%)
100
92
90
1:8
Range (%)
92-106
82-93
86-99
Average (%)
98
88
91
1:16
Range (%)
87-101
83-97
82-94
Average (%)
93
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
92-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.67
14.04
46.36
4.5
12.87
44.46
Standard deviation
0.32
0.79
2.48
0.27
0.7
2.01
C V (%)
6.85
5.63
5.35
6.0
5.44
4.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse COMP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse COMP in samples. No significant cross-reactivity or interference between Mouse COMP and analogues was observed.
