The Mouse CP (Ceruloplasmin) CLIA Kit is specifically designed for the precise measurement of ceruloplasmin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are essential for a variety of research purposes.Ceruloplasmin is a vital protein that plays a crucial role in iron metabolism, oxidative stress response, and inflammation regulation.
As such, it is a key biomarker for studying conditions such as Wilson's disease, Alzheimer's disease, and cardiovascular disorders. The Mouse CP CLIA Kit provides researchers with a valuable tool for investigating these conditions and developing potential therapeutic interventions.
Product Name:
Mouse CP (Ceruloplasmin) CLIA Kit
SKU:
MOES00162
Target:
Mouse CP (Ceruloplasmin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CP. You can calculate the concentration of Mouse CP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
97-110
89-103
Average (%)
97
103
97
1:4
Range (%)
104-120
101-118
89-100
Average (%)
110
107
94
1:8
Range (%)
88-101
90-105
96-108
Average (%)
94
97
103
1:16
Range (%)
92-106
90-103
93-106
Average (%)
98
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.82
4.74
19.6
1.66
4.5
17.77
Standard deviation
0.16
0.34
1.9
0.2
0.33
1.12
C V (%)
8.79
7.17
9.69
12.05
7.33
6.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CP in samples. No significant cross-reactivity or interference between Mouse CP and analogues was observed.
