The Mouse CTSK (Cathepsin K) CLIA Kit is specifically designed for precise measurement of Cathepsin K levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.Cathepsin K is a key enzyme involved in bone remodeling and degradation of extracellular matrix proteins.
It plays a crucial role in processes such as osteoclast-mediated bone resorption and has been linked to conditions like osteoporosis and arthritis. Monitoring Cathepsin K levels can provide valuable insights into bone health and potential therapeutic targets for related diseases.
Product Name:
Mouse CTSK (Cathepsin K) CLIA Kit
SKU:
MOES00159
Target:
Mouse CTSK (Cathepsin K)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTSK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTSK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CTSK. You can calculate the concentration of Mouse CTSK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
83-99
103-120
Average (%)
102
90
109
1:4
Range (%)
102-117
96-113
103-116
Average (%)
107
103
109
1:8
Range (%)
95-106
99-113
93-105
Average (%)
100
107
98
1:16
Range (%)
90-101
88-100
94-107
Average (%)
95
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.58
65.17
199.95
25.56
68.21
212.97
Standard deviation
2.27
6.35
14.58
3.03
5.14
20.36
C V (%)
8.87
9.74
7.29
11.85
7.54
9.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CTSK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTSK in samples. No significant cross-reactivity or interference between Mouse CTSK and analogues was observed.
