The Mouse CTSK (Cathepsin K) ELISA Kit is specifically designed for the accurate quantification of Cathepsin K levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Cathepsin K is a critical enzyme involved in bone resorption and remodeling, making it a key target for studying conditions such as osteoporosis, arthritis, and other bone-related disorders.
By measuring Cathepsin K levels, researchers can gain valuable insights into disease progression and potential therapeutic interventions.With its advanced technology and user-friendly protocol, the Mouse CTSK ELISA Kit from Assay Genie is an essential tool for investigating the role of Cathepsin K in various biological processes and disease conditions. Trust in the accuracy and precision of this kit to advance your research and discovery efforts.
Product Name:
Mouse CTSK (Cathepsin K) ELISA Kit
SKU:
MOES00820
Target:
Mouse CTSK (Cathepsin K)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTSK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTSK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CTSK. You can calculate the concentration of Mouse CTSK in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
95-107
83-97
Average (%)
104
100
90
1:4
Range (%)
89-102
84-96
88-103
Average (%)
94
91
95
1:8
Range (%)
90-106
88-100
84-98
Average (%)
97
93
90
1:16
Range (%)
87-103
82-95
86-102
Average (%)
94
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
93-103
98
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.87
135.85
457.69
53.87
133.39
422.39
Standard deviation
3.53
5.83
24.49
3.52
5.44
18.5
C V (%)
6.81
4.29
5.35
6.53
4.08
4.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse CTSK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTSK in samples. No significant cross-reactivity or interference between Mouse CTSK and analogues was observed.
