The Mouse CTSL (Cathepsin L) CLIA Kit is a highly sensitive assay designed for the accurate detection of Cathepsin L levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a variety of research applications.Cathepsin L is a key enzyme involved in various cellular processes, including protein degradation, tissue remodeling, and immune response modulation. Dysregulation of Cathepsin L has been implicated in diseases such as cancer, autoimmune disorders, and osteoporosis, highlighting its importance as a potential biomarker for disease progression and treatment assessment.
With its high sensitivity and specificity, the Mouse CTSL CLIA Kit is an essential tool for researchers studying Cathepsin L biology and its role in disease pathology. Get reliable and accurate results with this advanced assay kit from AssayGenie.
Product Name:
Mouse CTSL (Cathepsin L) CLIA Kit
SKU:
MOES00160
Target:
Mouse CTSL (Cathepsin L)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTSL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTSL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CTSL. You can calculate the concentration of Mouse CTSL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
93-106
101-117
Average (%)
103
99
109
1:4
Range (%)
100-118
85-100
90-102
Average (%)
108
92
96
1:8
Range (%)
102-118
85-98
89-103
Average (%)
110
91
97
1:16
Range (%)
86-101
98-115
92-107
Average (%)
92
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
101-117
108
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.96
126.48
353.86
46.96
115.3
347.21
Standard deviation
3.94
11.42
27.78
4.64
13.65
29.2
C V (%)
8.96
9.03
7.85
9.88
11.84
8.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CTSL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CTSL in samples. No significant cross-reactivity or interference between Mouse CTSL and analogues was observed.
