The Mouse CYP7A1 (Cytochrome P450 7A1) CLIA Kit is specifically designed for the accurate detection of CYP7A1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CYP7A1 is a key enzyme involved in the metabolism of cholesterol and bile acid synthesis in the liver. Dysregulation of CYP7A1 has been implicated in various metabolic disorders, including dyslipidemia and cholestatic liver diseases.
The ability to accurately measure CYP7A1 levels in mouse samples is critical for understanding the mechanisms underlying these conditions and exploring potential therapeutic interventions.Overall, the Mouse CYP7A1 CLIA Kit offers a valuable tool for researchers studying lipid metabolism, liver function, and related metabolic disorders in mouse models. Its high performance and ease of use make it a reliable choice for precise quantification of CYP7A1 levels in biological samples.
Product Name:
Mouse CYP7A1 (Cytochrome P450 7A1) CLIA Kit
SKU:
MOES00218
Target:
Mouse CYP7A1 (Cytochrome P450 7A1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYP7A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CYP7A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYP7A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CYP7A1. You can calculate the concentration of Mouse CYP7A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
99-114
98-111
Average (%)
97
106
104
1:4
Range (%)
95-107
100-118
98-113
Average (%)
101
108
105
1:8
Range (%)
89-104
94-106
99-115
Average (%)
96
100
105
1:16
Range (%)
95-110
101-119
100-117
Average (%)
101
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
101
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.39
483.68
1361.79
192.11
530.62
1442.14
Standard deviation
23.44
50.5
137.4
21.63
44.73
130.08
C V (%)
11.14
10.44
10.09
11.26
8.43
9.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CYP7A1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CYP7A1 in samples. No significant cross-reactivity or interference between Mouse CYP7A1 and analogues was observed.
