The Mouse CYT-C (Cytochrome C) CLIA Kit is specifically designed for the detection of cytochrome C levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, allowing for accurate and consistent results, making it an ideal tool for various research applications.Cytochrome C is a key protein involved in the electron transport chain, playing a critical role in cellular respiration. It is also known to be released from mitochondria during apoptosis, making it a valuable marker for studying cell death processes and evaluating mitochondrial function in various disease states.
With the Mouse CYT-C CLIA Kit, researchers can gain valuable insights into mitochondrial dysfunction, oxidative stress, and apoptosis pathways in mouse models, aiding in the development of novel therapies for diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
Product Name:
Mouse Cyt C (Cytochrome C) CLIA Kit
SKU:
MOES00020
Target:
Mouse Cyt C (Cytochrome C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Cyt-C. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Cyt-C, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Cyt-C. You can calculate the concentration of Mouse Cyt-C in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
94-107
90-104
Average (%)
94
101
97
1:4
Range (%)
85-95
92-106
99-111
Average (%)
90
98
105
1:8
Range (%)
87-100
90-104
89-106
Average (%)
94
95
97
1:16
Range (%)
98-110
85-98
90-105
Average (%)
103
92
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
99-114
105
Cell culture media (n=5)
88-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
237.37
450.09
1987.83
214.19
491.2
1795.63
Standard deviation
19.65
51.58
189.24
21.01
42.83
151.19
C V (%)
8.28
11.46
9.52
9.81
8.72
8.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Cyt-C concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Cyt-C in samples. No significant cross-reactivity or interference between Mouse Cyt-C and analogues was observed.
