The Mouse DAO (Diamine Oxidase) CLIA Kit is specifically designed for the precise measurement of diamine oxidase levels in mouse serum, plasma, and tissue samples. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an ideal tool for a variety of research purposes.Diamine oxidase is an important enzyme involved in the metabolism of histamine, playing a critical role in the regulation of allergic reactions and immune responses.
Abnormal levels of DAO have been linked to conditions such as food intolerances, gastrointestinal disorders, and histamine intolerance, highlighting the significance of studying this enzyme in disease pathology and treatment.This Mouse DAO CLIA Kit provides researchers with a reliable and efficient method for measuring diamine oxidase levels in mouse samples, offering valuable insights into the role of this enzyme in various physiological and pathological processes.
Product Name:
Mouse DAO (Diamine Oxidase) CLIA Kit
SKU:
MOES00224
Target:
Mouse DAO (Diamine Oxidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DAO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DAO. You can calculate the concentration of Mouse DAO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
88-103
96-112
Average (%)
107
94
102
1:4
Range (%)
100-116
98-114
101-113
Average (%)
106
104
106
1:8
Range (%)
87-97
99-112
91-108
Average (%)
92
106
98
1:16
Range (%)
97-110
95-107
96-111
Average (%)
102
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
104
EDTA plasma (n=5)
96-109
101
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.3
6.81
21.75
2.37
7.42
22.19
Standard deviation
0.21
0.72
2.46
0.2
0.72
2.48
C V (%)
9.13
10.57
11.31
8.44
9.7
11.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DAO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DAO in samples. No significant cross-reactivity or interference between Mouse DAO and analogues was observed.
