Mouse Deiodinase, Iodothyronine, Type III (DIO3) ELISA Kit
The Mouse Deiodinase Iodothyronine Type III (DIO3) ELISA Kit is specifically designed for the accurate measurement of DIO3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.DIO3 is an important enzyme involved in the regulation of thyroid hormone levels and metabolism. It plays a critical role in thyroid hormone signaling and has been implicated in various physiological processes such as metabolism, thermogenesis, and development.
By accurately measuring DIO3 levels, researchers can gain valuable insights into the function and regulation of thyroid hormone metabolism in mice.Overall, the Mouse Deiodinase Iodothyronine Type III (DIO3) ELISA Kit is a powerful tool for studying thyroid hormone metabolism and its impact on various physiological processes in mouse models. Its high sensitivity and specificity make it an essential resource for researchers looking to investigate DIO3 levels in a reliable and reproducible manner.
Product Name:
Mouse Deiodinase, Iodothyronine, Type III (DIO3) ELISA Kit
Product Code:
MODL00373
Size:
96 Assays
Target:
DIO3
Synonyms:
ITDI3, TXDI3, Type-III 5'-deiodinase
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
