The Mouse DKK1 (Dickkopf-related protein 1) CLIA Kit is specially designed for the quantification of DKK1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.DKK1 is a key regulator of the Wnt signaling pathway, playing a critical role in cell differentiation, proliferation, and apoptosis. Dysregulation of DKK1 has been implicated in numerous diseases, including cancer, osteoporosis, and Alzheimer's disease, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its user-friendly protocol and robust performance, the Mouse DKK1 CLIA Kit is an essential tool for researchers seeking to investigate the role of DKK1 in disease pathology and identify novel therapeutic targets.
Product Name:
Mouse DKK1 (Dickkopf Related Protein 1) CLIA Kit
SKU:
MOES00021
Target:
Mouse DKK1 (Dickkopf Related Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DKK1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DKK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DKK1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DKK1. You can calculate the concentration of Mouse DKK1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
92-103
103-117
Average (%)
105
97
110
1:4
Range (%)
98-109
97-114
85-96
Average (%)
103
105
90
1:8
Range (%)
97-111
98-112
87-97
Average (%)
105
106
92
1:16
Range (%)
97-111
101-114
83-98
Average (%)
104
108
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
99-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.21
108.31
462.79
46.21
104.84
421.41
Standard deviation
4.96
10.26
36.51
5.97
11.73
42.06
C V (%)
10.51
9.47
7.89
12.92
11.19
9.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DKK1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DKK1 in samples. No significant cross-reactivity or interference between Mouse DKK1 and analogues was observed.
