The Mouse DKK2 (Dickkopf-related protein 2) CLIA Kit is a powerful tool for measuring DKK2 levels in mouse serum, plasma, and tissue homogenates. This kit is highly sensitive and specific, providing accurate and consistent results for various research applications.DKK2 is a key regulator of the Wnt signaling pathway, playing a critical role in embryonic development, tissue regeneration, and cancer progression. Dysregulation of DKK2 has been linked to various diseases, making it a valuable biomarker for studying disease mechanisms and developing targeted therapies.
With its advanced technology and user-friendly protocol, the Mouse DKK2 CLIA Kit is a reliable solution for researchers looking to explore the role of DKK2 in health and disease. Get precise and reproducible results with this cutting-edge kit from Assay Genie.
Product Name:
Mouse DKK2 (Dickkopf Related Protein 2) CLIA Kit
SKU:
MOES00225
Target:
Mouse DKK2 (Dickkopf Related Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DKK2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DKK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DKK2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DKK2. You can calculate the concentration of Mouse DKK2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
87-101
102-120
Average (%)
107
93
110
1:4
Range (%)
96-111
86-97
89-102
Average (%)
104
91
94
1:8
Range (%)
99-114
88-99
98-114
Average (%)
106
94
104
1:16
Range (%)
93-110
89-100
90-102
Average (%)
100
94
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
96-109
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.87
54.84
140.58
19.59
57.03
144.19
Standard deviation
2.24
6.29
8.6
2.32
4.98
14.33
C V (%)
11.87
11.47
6.12
11.84
8.73
9.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DKK2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DKK2 in samples. No significant cross-reactivity or interference between Mouse DKK2 and analogues was observed.
