The Mouse DKK3 (Dickkopf-related protein 3) ELISA Kit is specifically designed for the accurate detection of DKK3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.DKK3 is a key player in regulating the Wnt signaling pathway and has been implicated in various diseases including cancer, cardiovascular diseases, and neurodegenerative disorders.
By studying DKK3 levels, researchers can gain insights into the underlying mechanisms of these conditions and potentially develop targeted therapies.With its user-friendly format and reliable performance, the Mouse DKK3 ELISA Kit from Assay Genie is a valuable tool for researchers looking to study the role of DKK3 in disease pathology and therapeutic interventions.
Product Name:
Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit
SKU:
MOES00977
Target:
Mouse DKK3 (Dickkopf Related Protein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DKK3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DKK3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DKK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse DKK3. You can calculate the concentration of Mouse DKK3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
93-108
99-114
Average (%)
91
99
106
1:4
Range (%)
87-100
85-98
80-95
Average (%)
93
90
87
1:8
Range (%)
91-107
84-98
82-93
Average (%)
98
91
88
1:16
Range (%)
90-104
81-93
85-96
Average (%)
96
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
97
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.37
91.37
432.91
48.38
82.45
391.8
Standard deviation
2.71
4.79
22.04
3.12
3.68
11.99
C V (%)
5.08
5.24
5.09
6.45
4.46
3.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse DKK3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DKK3 in samples. No significant cross-reactivity or interference between Mouse DKK3 and analogues was observed.
