The Mouse ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit is a specialized assay designed for the quantitative measurement of ECF, also known as CCL11, in mouse biological samples. ECF is a critical chemokine involved in eosinophil recruitment and activation processes, playing a key role in various inflammatory responses, particularly those involving allergic reactions and immune modulation. This ELISA kit offers exceptional sensitivity and specificity, enabling researchers to accurately quantify ECF levels in biological samples for investigative studies related to eosinophil function, allergic diseases, and inflammatory conditions.
With stringent quality control measures, the kit ensures robust and reliable performance, delivering reproducible results. Its user-friendly design makes it an excellent choice for preclinical research and studies focused on understanding immune responses and inflammatory pathways.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ECF/CCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ECF/CCL11. You can calculate the concentration of Mouse ECF/CCL11 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-102
89-99
82-98
Average (%)
95
92
90
1:4
Range (%)
95-108
92-102
97-109
Average (%)
103
97
104
1:8
Range (%)
87-99
99-107
82-95
Average (%)
95
102
90
1:16
Range (%)
90-102
82-94
89-100
Average (%)
94
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-108
105
EDTA plasma (n=5)
92-105
98
Cell culture media (n=5)
92-103
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
167.16
438.75
1630.13
171.09
463.75
1723.54
Standard deviation
8.68
21.54
66.51
9.96
21.15
71.53
C V (%)
5.19
4.91
4.08
5.82
4.56
4.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ECF/CCL11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ECF/CCL11 in samples. No significant cross-reactivity or interference between Mouse ECF/CCL11 and analogues was observed.
