Mouse EGR1 (Early Growth Response Protein 1) CLIA Kit
The Mouse EGR1 (Early Growth Response Protein 1) CLIA Kit is specially designed for the accurate and reliable detection of EGR1 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise results for a variety of research applications.EGR1 is a key transcription factor that plays a critical role in regulating the expression of genes involved in cell growth, differentiation, and response to stress.
Its dysregulation has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions, making it a valuable biomarker for studying these diseases and developing targeted therapies.Overall, the Mouse EGR1 CLIA Kit provides researchers with a powerful tool to investigate the role of EGR1 in disease pathogenesis and explore its potential as a therapeutic target.
Product Name:
Mouse EGR1 (Early Growth Response Protein 1) CLIA Kit
SKU:
MOES00233
Target:
Mouse EGR1 (Early Growth Response Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EGR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse EGR1. You can calculate the concentration of Mouse EGR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
98-115
89-100
Average (%)
101
106
94
1:4
Range (%)
89-104
91-103
86-99
Average (%)
96
96
91
1:8
Range (%)
96-112
96-111
101-117
Average (%)
103
103
109
1:16
Range (%)
86-98
90-106
97-112
Average (%)
91
98
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
106
EDTA plasma (n=5)
95-108
100
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.83
301.66
744.82
98.54
314.2
686.16
Standard deviation
11.67
29.2
48.93
12.44
31.48
67.04
C V (%)
11.46
9.68
6.57
12.62
10.02
9.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse EGR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EGR1 in samples. No significant cross-reactivity or interference between Mouse EGR1 and analogues was observed.
