Mouse Elastin Microfibril Interface Located Protein 2 (EMILIN2) ELISA Kit
The Mouse EMILIN2 (Elastin microfibril interface-located protein 2) ELISA Kit is a specialized assay designed for the precise measurement of EMILIN2 levels in mouse biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications. EMILIN2 is a key protein involved in maintaining the integrity of the extracellular matrix and regulating tissue remodeling. It plays a crucial role in processes such as cell adhesion, migration, and differentiation, making it a valuable biomarker for studying various physiological and pathological conditions.
With its advanced technology and reliable performance, the Mouse EMILIN2 ELISA Kit is an essential tool for researchers investigating the role of EMILIN2 in diseases such as cancer, cardiovascular disorders, and fibrosis, and for developing potential therapeutic interventions. Upgrade your research with this cutting-edge kit and unlock new insights into the complex mechanisms of EMILIN2 function.
Product Name:
Mouse Elastin Microfibril Interface Located Protein 2 (EMILIN2) ELISA Kit
Product Code:
MODL00419
Size:
96 Assays
Target:
EMILIN2
Synonyms:
FOAP-10, Elastin Microfibril Interfacer 2
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
