The Mouse ELN (Elastin) CLIA Kit is specifically designed to accurately measure elastin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for various research applications.Elastin is a vital protein that plays a key role in maintaining tissue elasticity and structure. Changes in elastin levels have been linked to numerous health conditions, including cardiovascular diseases, skin disorders, and lung diseases.
Therefore, studying elastin levels can provide essential insights into these conditions and potential therapeutic strategies.For researchers working with mouse models, the Mouse ELN CLIA Kit offers a comprehensive solution for measuring elastin levels with precision and efficiency. Trust in this kit to deliver accurate results and advance your research in the field of elastin biology.
Product Name:
Mouse ELN (Elastin) CLIA Kit
SKU:
MOES00237
Target:
Mouse ELN (Elastin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ELN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ELN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ELN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ELN. You can calculate the concentration of Mouse ELN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
101-116
95-108
Average (%)
102
109
100
1:4
Range (%)
100-114
103-117
95-108
Average (%)
108
110
101
1:8
Range (%)
95-110
100-115
94-108
Average (%)
102
107
101
1:16
Range (%)
84-98
88-99
90-102
Average (%)
91
94
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
92-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.67
267.12
885.23
98.31
289.99
839.02
Standard deviation
12.13
18.89
66.75
10.78
28.13
83.65
C V (%)
12.68
7.07
7.54
10.97
9.7
9.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ELN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ELN in samples. No significant cross-reactivity or interference between Mouse ELN and analogues was observed.
