The Mouse ENG (Endoglin) CLIA Kit is a highly reliable and accurate assay for the quantification of endoglin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Endoglin is a key protein involved in angiogenesis and vascular development, playing a critical role in numerous physiological and pathological processes.
Dysregulation of endoglin expression has been implicated in various diseases, including cancer, cardiovascular disorders, and fibrotic conditions, making it a valuable biomarker for studying disease progression and evaluating treatment efficacy.Overall, the Mouse ENG (Endoglin) CLIA Kit is an essential tool for researchers seeking to investigate the role of endoglin in disease pathogenesis and develop novel therapeutic strategies.
Product Name:
Mouse ENG (Endoglin) CLIA Kit
SKU:
MOES00532
Target:
Mouse ENG (Endoglin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ENG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ENG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ENG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ENG. You can calculate the concentration of Mouse ENG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
95-113
88-100
Average (%)
98
103
93
1:4
Range (%)
101-117
87-99
86-96
Average (%)
108
92
91
1:8
Range (%)
98-115
100-118
90-105
Average (%)
105
109
96
1:16
Range (%)
90-105
93-107
95-107
Average (%)
96
99
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
97-111
103
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.16
110.62
373.66
46.71
117.42
340.95
Standard deviation
5.78
9.41
25.0
5.04
8.99
34.78
C V (%)
12.52
8.51
6.69
10.79
7.66
10.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ENG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ENG in samples. No significant cross-reactivity or interference between Mouse ENG and analogues was observed.
