The Mouse EPO (Erythropoietin) ELISA Kit is a sophisticated tool designed to quantitatively measure Erythropoietin levels in a variety of biological samples with accuracy and precision. Erythropoietin, a pivotal hormone primarily secreted by the kidneys, plays a crucial role in regulating red blood cell production, also known as erythropoiesis. Accurate quantification of Erythropoietin is essential for investigating its involvement in erythropoiesis, anemia, and hematological disorders. As a vital regulator of red blood cell production, understanding Erythropoietin levels provides valuable insights into hematopoiesis and erythrocyte function. Through the use of the Mouse EPO ELISA Kit, researchers can unlock crucial information regarding Erythropoietin's function in hematological processes and anemia, paving the way for the development of targeted therapeutic interventions.
The Mouse EPO ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible results. Manufactured under strict quality control guidelines, this ELISA kit delivers consistent and robust performance, making it an ideal choice for researchers aiming to explore the role of Erythropoietin in hematopoiesis and hematologic disorders.
Product Name:
Mouse EPO (Erythropoietin) ELISA Kit
SKU:
AEES00249
Target:
Mouse EPO (Erythropoietin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse EPO. You can calculate the concentration of Mouse EPO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
90-104
94-109
Average (%)
99
96
102
1:4
Range (%)
92-106
86-98
94-110
Average (%)
99
92
102
1:8
Range (%)
93-109
86-99
92-106
Average (%)
100
93
99
1:16
Range (%)
91-103
90-103
98-112
Average (%)
98
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.13
53.05
201.16
23.16
55.54
200.89
Standard deviation
1.47
2.67
9.84
1.26
2.35
10.33
C V (%)
5.83
5.03
4.89
5.46
4.24
5.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse EPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EPO in samples. No significant cross-reactivity or interference between Mouse EPO and analogues was observed.
