The Mouse ER Alpha (Estrogen Receptor Alpha) CLIA Kit is specifically designed for the precise detection of ER Alpha levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.ER Alpha is a key protein involved in mediating estrogen signaling, regulating gene expression, and influencing cell growth and differentiation. It plays a critical role in various physiological processes, including development, reproduction, and metabolism. Dysregulation of ER Alpha has been implicated in numerous diseases, such as breast cancer, osteoporosis, and cardiovascular disorders, highlighting its importance as a target for therapeutic interventions.
The Mouse ER Alpha CLIA Kit provides researchers with a valuable tool for studying the role of ER Alpha in health and disease, enabling the exploration of new treatment strategies and diagnostic approaches. Its reliable performance and user-friendly format make it an ideal choice for laboratories conducting studies on estrogen receptor signaling pathways and related biological processes.
Product Name:
Mouse ER alpha (Estrogen Receptor Alpha) CLIA Kit
SKU:
MOES00250
Target:
Mouse ER alpha (Estrogen Receptor Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ERα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ERα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ERα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ERα. You can calculate the concentration of Mouse ERα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
85-98
97-113
Average (%)
100
91
104
1:4
Range (%)
91-105
99-117
90-103
Average (%)
99
107
97
1:8
Range (%)
93-110
86-101
99-114
Average (%)
100
92
106
1:16
Range (%)
89-101
84-99
100-111
Average (%)
96
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.86
518.67
1894.66
178.39
525.64
1866.66
Standard deviation
19.42
46.21
143.99
16.3
47.41
213.17
C V (%)
10.07
8.91
7.6
9.14
9.02
11.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ERα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ERα in samples. No significant cross-reactivity or interference between Mouse ERα and analogues was observed.
