The Mouse FGF21 (Fibroblast Growth Factor 21) CLIA Kit is a high-quality assay kit designed for the accurate detection of FGF21 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.FGF21 is a vital growth factor that plays a key role in regulating metabolism, glucose homeostasis, and energy balance.
It is associated with various metabolic disorders such as obesity, diabetes, and fatty liver disease, making it a crucial biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse FGF21 CLIA Kit is a valuable tool for researchers looking to investigate the role of FGF21 in metabolic disorders and explore its potential as a target for novel treatment strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF21. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FGF21. You can calculate the concentration of Mouse FGF21 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
94-106
97-111
Average (%)
104
101
103
1:4
Range (%)
102-119
100-114
97-110
Average (%)
109
108
104
1:8
Range (%)
86-96
86-102
93-106
Average (%)
91
93
99
1:16
Range (%)
85-98
84-97
100-115
Average (%)
91
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.27
91.46
330.05
41.19
92.94
360.27
Standard deviation
3.64
10.28
20.1
5.13
7.31
28.68
C V (%)
9.51
11.24
6.09
12.45
7.87
7.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FGF21 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGF21 in samples. No significant cross-reactivity or interference between Mouse FGF21 and analogues was observed.
