Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand) CLIA Kit
The Mouse FLT3L (Fms-like tyrosine kinase 3 ligand) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of FLT3L levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.FLT3L is a crucial cytokine that plays a key role in regulating hematopoietic stem cell proliferation and differentiation.
It is also involved in immune cell development and function, making it a valuable biomarker for studying immune responses, hematopoiesis, and immune-related diseases.Whether studying hematopoiesis, immune responses, or the role of FLT3L in disease pathology, the Mouse FLT3L CLIA Kit offers researchers a powerful tool for accurate and precise measurement of FLT3L levels in mouse samples.
Product Name:
Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand) CLIA Kit
SKU:
MOES00027
Target:
Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Flt3L. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Flt3L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Flt3L, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Flt3L. You can calculate the concentration of Mouse Flt3L in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
102-118
94-106
Average (%)
93
109
100
1:4
Range (%)
96-109
93-107
90-103
Average (%)
101
100
96
1:8
Range (%)
90-103
98-109
96-113
Average (%)
98
104
103
1:16
Range (%)
85-97
93-109
86-101
Average (%)
92
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
97
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.15
67.85
210.72
26.35
64.54
223.05
Standard deviation
3.02
5.12
19.18
2.46
5.67
21.35
C V (%)
12.51
7.55
9.1
9.34
8.79
9.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Flt3L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Flt3L in samples. No significant cross-reactivity or interference between Mouse Flt3L and analogues was observed.
