The Assay Genie Mouse Flt3L (FMS-like Tyrosine Kinase 3 Ligand) ELISA Kit is meticulously crafted to allow the precise quantification of Flt3L levels in various biological samples derived from mouse models. Flt3L, a critical growth factor pivotal in hematopoiesis and immune cell development, plays a vital role in regulating the differentiation, proliferation, and survival of hematopoietic stem cells and dendritic cells. Accurate measurement of Flt3L is crucial for understanding its role in immune modulation, hematopoiesis, and disease pathogenesis. Our Flt3L ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible results for your research investigations.
Manufactured under stringent quality control processes, this kit delivers consistent performance and user-friendly protocols that facilitate ease of use, making it an excellent research tool for studying immune responses, hematopoiesis, and related biological processes. Trust Assay Genie's Flt3L ELISA Kit for precise and dependable quantification of FMS-like Tyrosine Kinase 3 Ligand in your mouse model studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Flt3L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Flt3L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Flt3L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Flt3L. You can calculate the concentration of Mouse Flt3L in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
96-109
90-103
Average (%)
105
103
95
1:4
Range (%)
87-101
82-94
83-97
Average (%)
94
89
89
1:8
Range (%)
88-99
79-93
90-100
Average (%)
93
86
95
1:16
Range (%)
91-104
82-94
82-92
Average (%)
98
87
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.07
90.37
413.18
52.77
94.12
389.66
Standard deviation
2.94
3.67
20.49
3.26
3.94
19.29
C V (%)
6.12
4.06
4.96
6.18
4.19
4.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Flt3L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Flt3L in samples. No significant cross-reactivity or interference between Mouse Flt3L and analogues was observed.
