The Mouse FV (Coagulation Factor V) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of Coagulation Factor V in mouse serum, plasma, and other biological samples. This kit boasts high sensitivity and specificity, providing dependable and consistent results that are ideal for a variety of research applications.Coagulation Factor V is a key player in the blood clotting process, essential for maintaining hemostasis and preventing excessive bleeding.
Dysregulation of Coagulation Factor V has been linked to various bleeding disorders and thrombotic events, underscoring its importance as a biomarker for studying these conditions and potential therapeutic interventions.With the Mouse FV CLIA Kit, researchers can gain valuable insights into the role of Coagulation Factor V in health and disease, paving the way for innovative research and the development of new treatment strategies.
Product Name:
Mouse FV (Coagulation Factor V) CLIA Kit
SKU:
MOES00182
Target:
Mouse FV (Coagulation Factor V)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FV. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FV and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FV, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FV. You can calculate the concentration of Mouse FV in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
99-114
101-117
Average (%)
106
105
108
1:4
Range (%)
96-113
98-112
89-100
Average (%)
104
104
94
1:8
Range (%)
100-111
99-117
87-100
Average (%)
105
108
93
1:16
Range (%)
98-113
93-108
88-102
Average (%)
104
99
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
105
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.9
4.73
17.7
1.99
4.41
17.67
Standard deviation
0.17
0.48
1.83
0.17
0.52
1.43
C V (%)
8.95
10.15
10.34
8.54
11.79
8.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FV concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FV in samples. No significant cross-reactivity or interference between Mouse FV and analogues was observed.
