The Mouse Gal7 (Galectin-7) CLIA Kit is a powerful tool for researchers looking to accurately measure galectin-7 levels in mouse samples. Galectin-7 is a key protein involved in various cellular processes, including cell adhesion, apoptosis, and immune response.This CLIA kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.
Galectin-7 has been implicated in numerous diseases, such as cancer, inflammation, and skin disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Mouse Gal7 (Galectin-7) CLIA Kit from Assay Genie is a valuable addition to any research laboratory seeking to better understand the role of galectin-7 in health and disease.
Product Name:
Mouse GAL7 (Galectin 7) CLIA Kit
SKU:
MOES00279
Target:
Mouse GAL7 (Galectin 7)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL7. You can calculate the concentration of Mouse GAL7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
95-109
87-97
Average (%)
105
101
92
1:4
Range (%)
103-116
91-105
94-105
Average (%)
109
98
99
1:8
Range (%)
95-108
85-96
100-114
Average (%)
102
91
107
1:16
Range (%)
86-96
101-114
95-112
Average (%)
91
106
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
101-115
107
Cell culture media (n=5)
100-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.86
188.53
965.81
89.18
188.97
958.63
Standard deviation
11.79
20.91
99.29
8.67
16.91
62.31
C V (%)
12.83
11.09
10.28
9.72
8.95
6.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL7 in samples. No significant cross-reactivity or interference between Mouse GAL7 and analogues was observed.
