The Mouse GGT1 (Gamma-glutamyltransferase 1) CLIA Kit is specifically designed for the accurate detection of GGT1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for various research applications.GGT1 is an important enzyme involved in the metabolism of glutathione and other peptides, playing a critical role in maintaining cellular redox balance. Dysregulation of GGT1 has been linked to various diseases, such as liver disorders, kidney diseases, and certain types of cancer, making it a valuable biomarker for studying these conditions and developing potential treatments.
With its reliable performance and ease of use, the Mouse GGT1 CLIA Kit is an essential tool for researchers studying GGT1 function and its implications in health and disease. Get accurate and precise results with this advanced kit from AssayGenie.
Product Name:
Mouse gGT1 (Gamma Glutamyltransferase 1) CLIA Kit
SKU:
MOES00283
Target:
Mouse gGT1 (Gamma Glutamyltransferase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse gGT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse gGT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse gGT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse gGT1. You can calculate the concentration of Mouse gGT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
97-114
101-116
Average (%)
92
105
108
1:4
Range (%)
95-107
98-114
88-99
Average (%)
101
106
94
1:8
Range (%)
92-108
90-104
94-105
Average (%)
99
97
99
1:16
Range (%)
97-109
99-115
99-117
Average (%)
102
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-115
105
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.96
200.65
758.41
101.0
185.52
692.5
Standard deviation
12.16
23.07
46.41
11.98
16.73
69.32
C V (%)
11.93
11.5
6.12
11.86
9.02
10.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse gGT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse gGT1 in samples. No significant cross-reactivity or interference between Mouse gGT1 and analogues was observed.
