The Mouse GH (Growth Hormone) CLIA Kit is specifically designed for the precise quantification of growth hormone levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research purposes.Growth hormone is a key regulator of growth, metabolism, and development in mammals, playing a crucial role in various physiological processes. Dysregulation of growth hormone levels can lead to disorders such as dwarfism, gigantism, and metabolic abnormalities, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.
The Mouse GH CLIA Kit from Assay Genie provides researchers with a reliable tool for measuring growth hormone levels in mouse samples, facilitating in-depth investigations into the mechanisms underlying growth and development. Trust in our kit to deliver accurate and reliable results for your research needs.
Product Name:
Mouse GH (Growth Hormone) CLIA Kit
SKU:
MOES00052
Target:
Mouse GH (Growth Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GH. You can calculate the concentration of Mouse GH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
91-108
94-108
Average (%)
91
98
102
1:4
Range (%)
100-117
94-105
99-117
Average (%)
109
99
107
1:8
Range (%)
86-98
87-98
96-113
Average (%)
92
93
104
1:16
Range (%)
86-101
93-104
90-101
Average (%)
92
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
98-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
203.34
608.15
1950.46
186.32
551.64
1807.14
Standard deviation
22.9
72.55
163.25
18.84
52.52
159.03
C V (%)
11.26
11.93
8.37
10.11
9.52
8.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GH in samples. No significant cross-reactivity or interference between Mouse GH and analogues was observed.
