The Mouse Ghrelin ELISA Kit is specifically designed for the precise measurement of ghrelin levels in mouse serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it an ideal tool for various research applications. Ghrelin is a key hormone that regulates appetite and energy balance, playing a crucial role in metabolic processes and weight regulation.
It is also involved in the regulation of growth hormone release and gastric motility, making it a valuable biomarker for studying obesity, eating disorders, and metabolic diseases. By utilizing the Mouse Ghrelin ELISA Kit, researchers can effectively study the role of ghrelin in various physiological and pathological conditions, paving the way for potential therapeutic strategies targeting ghrelin signaling pathways.
Product Name:
Mouse GHRL (Ghrelin) ELISA Kit
SKU:
MOES01068
Target:
Mouse GHRL (Ghrelin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GHRL. During the reaction, GHRL in the sample or standard competes with a fixed amount of GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
96-108
96-111
Average (%)
103
103
102
1:4
Range (%)
89-101
91-103
96-108
Average (%)
95
97
102
1:8
Range (%)
90-102
87-100
99-117
Average (%)
96
94
107
1:16
Range (%)
91-105
91-103
98-116
Average (%)
96
97
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
90-107
97
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
3.8
0.5
1.3
3.5
Standard deviation
0.03
0.05
0.13
0.03
0.05
0.16
C V (%)
6
4.17
3.42
6.0
3.85
4.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GHRL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GHRL in samples. No significant cross-reactivity or interference between GHRL and analogues was observed.
