The Mouse GP130 (Glucoprotein 130) CLIA Kit is a powerful tool for detecting GP130 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for precise and consistent results, making it perfect for various research purposes.GP130 is a key protein that plays a critical role in cell signaling pathways, influencing various biological processes such as inflammation, immune response, and cell growth. Dysregulation of GP130 has been linked to a range of diseases, including cancer, inflammatory disorders, and metabolic syndromes, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its advanced technology and reliable performance, the Mouse GP130 CLIA Kit from Assay Genie provides researchers with a valuable tool for investigating the role of GP130 in disease pathogenesis and developing new treatment strategies.
Product Name:
Mouse gp130 (Glucoprotein 130) CLIA Kit
SKU:
MOES00294
Target:
Mouse gp130 (Glucoprotein 130)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse gp130. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse gp130 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse gp130, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse gp130. You can calculate the concentration of Mouse gp130 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
93-109
94-109
Average (%)
104
100
101
1:4
Range (%)
100-112
85-100
101-112
Average (%)
106
92
106
1:8
Range (%)
100-116
85-96
99-116
Average (%)
106
91
107
1:16
Range (%)
99-115
101-120
87-98
Average (%)
105
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
87-98
93
Cell culture media (n=5)
101-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.24
276.97
889.92
112.75
276.89
944.31
Standard deviation
9.03
22.46
75.91
10.18
28.96
88.39
C V (%)
8.66
8.11
8.53
9.03
10.46
9.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse gp130 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse gp130 in samples. No significant cross-reactivity or interference between Mouse gp130 and analogues was observed.
