The Mouse GREM1 (Gremlin-1) CLIA Kit is specifically designed for the precise measurement of Gremlin-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research experiments.Gremlin-1 is a key regulator in embryonic development and has been implicated in various diseases, including cancer and inflammatory conditions.
By accurately measuring Gremlin-1 levels, researchers can gain valuable insights into its role in disease progression and potentially identify new therapeutic targets.Overall, the Mouse GREM1 CLIA Kit is an essential tool for researchers studying the role of Gremlin-1 in health and disease, providing reliable data for further exploration and potential clinical applications.
Product Name:
Mouse GREM1 (GremLin 1) CLIA Kit
SKU:
MOES00313
Target:
Mouse GREM1 (GremLin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GREM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GREM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GREM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GREM1. You can calculate the concentration of Mouse GREM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
88-101
101-118
Average (%)
95
94
109
1:4
Range (%)
86-98
99-112
91-106
Average (%)
92
105
97
1:8
Range (%)
95-112
95-109
88-101
Average (%)
102
101
94
1:16
Range (%)
103-116
88-99
97-112
Average (%)
109
94
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
92-106
97
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.2
147.45
395.85
50.57
137.78
401.37
Standard deviation
5.3
10.37
43.15
4.53
13.19
33.47
C V (%)
11.47
7.03
10.9
8.96
9.57
8.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GREM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GREM1 in samples. No significant cross-reactivity or interference between Mouse GREM1 and analogues was observed.
