The Mouse Gro-Alpha (CXCL1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Gro-Alpha levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Gro-Alpha, also known as Growth-Regulated Oncogene Alpha, is a key protein involved in inflammation and immune responses. It plays a crucial role in conditions such as cancer, inflammatory diseases, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.
Overall, the Mouse Gro-Alpha (CXCL1) CLIA Kit is essential for researchers looking to understand the role of Gro-Alpha in various physiological and pathological processes in mice, providing valuable insights for future research and therapeutic development.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GROα/CXCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GROα/CXCL1. You can calculate the concentration of Mouse GROα/CXCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
87-98
101-117
Average (%)
108
92
108
1:4
Range (%)
94-110
100-116
89-99
Average (%)
100
107
94
1:8
Range (%)
100-115
91-107
93-106
Average (%)
106
98
99
1:16
Range (%)
85-97
100-118
104-121
Average (%)
91
108
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-110
100
EDTA plasma (n=5)
91-107
97
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.43
71.1
222.79
25.36
69.65
209.34
Standard deviation
2.68
7.6
22.55
2.72
5.2
16.16
C V (%)
11.44
10.69
10.12
10.73
7.47
7.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GROα/CXCL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GROα/CXCL1 in samples. No significant cross-reactivity or interference between Mouse GROα/CXCL1 and analogues was observed.
