The Mouse GRO-β (Growth-regulated Oncogene Beta) CLIA Kit is specifically designed for efficient and precise detection of GRO-β levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research applications.GRO-β, also known as CXCL2, is a critical chemokine involved in inflammation and immune responses, playing a crucial role in recruiting neutrophils to sites of infection or injury.
Dysregulation of GRO-β has been implicated in various diseases, including cancer and inflammatory disorders, underscoring its importance as a biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse GRO-β CLIA Kit offers researchers a reliable tool for studying the role of GRO-β in physiological and pathological processes, providing valuable insights into its functions and potential therapeutic applications.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GROβ/CXCL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GROβ/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GROβ/CXCL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GROβ/CXCL2. You can calculate the concentration of Mouse GROβ/CXCL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
98-114
86-101
Average (%)
104
104
93
1:4
Range (%)
85-97
100-114
103-116
Average (%)
92
108
109
1:8
Range (%)
86-96
86-98
87-98
Average (%)
91
91
93
1:16
Range (%)
103-117
87-100
92-105
Average (%)
109
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
100-117
107
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.5
53.77
188.66
26.68
51.65
204.86
Standard deviation
2.33
4.69
13.51
2.62
4.86
22.88
C V (%)
9.51
8.72
7.16
9.82
9.41
11.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GROβ/CXCL2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Mouse GROβ/CXCL2 and analogues was observed.
