The Mouse Gro-beta (Growth Regulated Oncogene Beta) ELISA Kit is designed for the precise measurement of Gro-beta levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring consistent and accurate results, which makes it perfect for a variety of research applications.Gro-beta, also known as CXCL2, is a key chemokine involved in inflammation and immune response, playing a crucial role in recruiting neutrophils to sites of infection or injury.
It is implicated in various inflammatory conditions, making it a valuable biomarker for studying these diseases and potentially developing targeted therapies. For researchers looking to investigate the role of Gro-beta in immune response and inflammation, the Mouse Gro-beta ELISA Kit offers a reliable and efficient solution for detecting and quantifying this important protein in mouse samples.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GROβ/CXCL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GROβ/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GROβ/CXCL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GROβ/CXCL2. You can calculate the concentration of Mouse GROβ/CXCL2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
89-103
91-104
Average (%)
92
97
97
1:4
Range (%)
95-110
86-98
95-111
Average (%)
102
92
103
1:8
Range (%)
99-116
82-96
95-110
Average (%)
106
88
102
1:16
Range (%)
102-116
88-100
95-108
Average (%)
108
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
89-102
97
Cell culture media (n=5)
83-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.8
121.2
461.5
48.6
121.4
496.4
Standard deviation
3.3
5.9
16.2
2.6
5.2
23.3
C V (%)
6.9
4.87
3.51
5.35
4.28
4.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GROβ/CXCL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Mouse GROβ/CXCL2 and analogues was observed.
