Mouse GRP78 (Glucose Regulated Protein 78) ELISA Kit
The Mouse GRP78 (Glucose Regulated Protein 78) ELISA Kit is specifically designed for the accurate measurement of GRP78 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.GRP78 is a key protein involved in the regulation of glucose metabolism and cellular stress response. It plays a crucial role in multiple physiological and pathological processes, including cancer, diabetes, and neurodegenerative diseases.
By accurately measuring GRP78 levels, researchers can gain valuable insights into these conditions and potentially develop novel therapeutic interventions.Overall, the Mouse GRP78 ELISA Kit from Assay Genie is a valuable tool for studying the role of GRP78 in different disease contexts and for advancing research in the field of molecular biology and biochemistry.
Product Name:
Mouse GRP78 (Glucose Regulated Protein 78) ELISA Kit
SKU:
MOES01830
Target:
Mouse GRP78 (Glucose Regulated Protein 78) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRP78. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRP78 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRP78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GRP78. You can calculate the concentration of Mouse GRP78 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
96-109
90-102
Average (%)
97
104
95
1:4
Range (%)
89-103
82-97
82-95
Average (%)
95
88
88
1:8
Range (%)
90-101
84-96
84-99
Average (%)
95
90
91
1:16
Range (%)
92-103
86-99
86-101
Average (%)
97
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.59
6.83
1.01
2.65
6.27
Standard deviation
0.06
0.13
0.33
0.06
0.13
0.23
C V (%)
6.38
5.02
4.83
5.94
4.91
3.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GRP78 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GRP78 in samples. No significant cross-reactivity or interference between Mouse GRP78 and analogues was observed.
