The Mouse Haase Hyaluronidase CLIA Kit is a cutting-edge tool designed for the precise measurement of Haase hyaluronidase levels in mouse samples. This advanced kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Haase hyaluronidase is a key enzyme responsible for breaking down hyaluronic acid, a vital component of the extracellular matrix. By degrading hyaluronic acid, Haase hyaluronidase plays a crucial role in various physiological processes, including tissue remodeling, wound healing, and inflammation.
Understanding the activity of this enzyme is crucial for studying a wide range of biological processes and diseases.Whether you are investigating the role of Haase hyaluronidase in cell signaling pathways, evaluating its potential as a therapeutic target, or exploring its implications in disease pathogenesis, the Mouse Haase Hyaluronidase CLIA Kit offers a reliable and efficient solution for your research needs. Trust in this innovative kit to deliver accurate and insightful data, advancing your scientific discoveries in the field of molecular biology and beyond.
Product Name:
Mouse HAase (Hyaluronidase) CLIA Kit
SKU:
MOES00373
Target:
Mouse HAase (Hyaluronidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HAase. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HAase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HAase, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HAase. You can calculate the concentration of Mouse HAase in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
98-114
95-109
Average (%)
105
104
103
1:4
Range (%)
87-101
99-111
88-102
Average (%)
93
104
95
1:8
Range (%)
95-110
96-110
92-108
Average (%)
100
103
99
1:16
Range (%)
99-112
85-98
89-101
Average (%)
105
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
100-114
106
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.59
7.24
23.05
2.49
7.48
21.05
Standard deviation
0.27
0.51
1.47
0.3
0.64
1.33
C V (%)
10.42
7.04
6.38
12.05
8.56
6.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HAase concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HAase in samples. No significant cross-reactivity or interference between Mouse HAase and analogues was observed.
