The Mouse Hemoglobin (HB) ELISA Kit is specifically designed for the quantitative detection of Hemoglobin levels in various mouse biological samples. Hemoglobin, a crucial protein found in red blood cells, plays a vital role in oxygen transport from the lungs to the tissues and carries carbon dioxide back to the lungs for removal.
This ELISA kit offers exceptional sensitivity and specificity, enabling accurate and reliable measurement of Hemoglobin levels in research samples. Manufactured under stringent quality control standards, the kit provides robust performance, ensuring precise results for studies related to hematological processes and blood disorders.
Product Name:
Mouse HB (Hemoglobin) ELISA Kit
SKU:
AEES00224
Target:
Mouse HB (Hemoglobin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
93.75 ng/mL
Detection range:
156.25-10000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HB. You can calculate the concentration of Mouse HB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-105
95-103
86-99
Average (%)
101
100
93
1:4
Range (%)
92-103
88-95
84-96
Average (%)
100
92
90
1:8
Range (%)
95-105
91-100
94-106
Average (%)
98
95
102
1:16
Range (%)
99-111
85-99
95-108
Average (%)
105
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
82-93
90
EDTA plasma (n=5)
85-95
92
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
485.08
1141.81
4717.73
417.18
1184.65
4067.68
Standard deviation
24.64
55.61
214.66
21.4
53.9
209.49
C V (%)
5.08
4.87
4.55
5.13
4.55
5.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse HB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HB in samples. No significant cross-reactivity or interference between Mouse HB and analogues was observed.
