The Mouse HDL (High-Density Lipoprotein) CLIA Kit is a cutting-edge assay kit designed for the precise measurement of HDL levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.HDL is a key player in lipid metabolism and cardiovascular health, known for its role in transporting cholesterol from peripheral tissues to the liver for excretion. Monitoring HDL levels is crucial for understanding the risk of cardiovascular diseases and assessing the efficacy of therapeutic interventions.
With its advanced technology and user-friendly format, the Mouse HDL CLIA Kit is an invaluable tool for researchers studying lipid metabolism, atherosclerosis, and related diseases in mouse models. Trust Assay Genie for high-quality reagents that deliver reliable data and drive groundbreaking discoveries in the field of biomedical research.
Product Name:
Mouse HDL (High Density Lipoprotein) CLIA Kit
SKU:
MOES00619
Target:
Mouse HDL (High Density Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 nmol/mL
Detection range:
0.63-40 nmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HDL. You can calculate the concentration of Mouse HDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
92-108
97-109
Average (%)
107
98
102
1:4
Range (%)
96-112
102-117
89-103
Average (%)
103
110
94
1:8
Range (%)
91-107
101-121
99-115
Average (%)
99
110
107
1:16
Range (%)
89-100
90-105
88-101
Average (%)
94
97
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
101-117
107
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.9
5.39
18.02
1.93
5.52
19.49
Standard deviation
0.17
0.52
1.43
0.22
0.47
1.56
C V (%)
8.95
9.65
7.94
11.4
8.51
8.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HDL in samples. No significant cross-reactivity or interference between Mouse HDL and analogues was observed.
