The Mouse Heme Oxygenase 1 (Decycling HO1) ELISA Kit is a reliable and efficient tool for measuring levels of heme oxygenase 1 in mouse samples including serum, plasma, and tissue homogenates. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Heme oxygenase 1 is a key enzyme involved in the breakdown of heme, playing a critical role in cellular response to oxidative stress and inflammation. Dysregulation of heme oxygenase 1 has been linked to various diseases including cancer, cardiovascular disorders, and neurodegenerative conditions.
Therefore, this ELISA kit is essential for studying the role of heme oxygenase 1 in disease pathogenesis and evaluating its potential as a therapeutic target.Overall, the Mouse Heme Oxygenase 1 (Decycling HO1) ELISA Kit is a valuable tool for researchers looking to investigate the physiological and pathological functions of heme oxygenase 1 in mouse models. Its high performance and reliability make it a great choice for advancing scientific inquiry and drug development efforts.
Product Name:
Mouse Heme Oxygenase 1, Decycling (HO1) ELISA Kit
Product Code:
MODL00626
Size:
96 Assays
Target:
HO1
Synonyms:
HMOX1, Hsp32, HMOX1D
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
