The Mouse HGF (Hepatocyte Growth Factor) CLIA Kit is a highly sensitive assay designed for the accurate detection of HGF levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and reliability, ensuring consistent and reproducible results for a variety of research applications.Hepatocyte Growth Factor is a key factor in cell growth, tissue regeneration, and wound healing, playing a crucial role in liver regeneration and development. It also has therapeutic potential in conditions such as liver diseases, cancer, and tissue repair, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and user-friendly protocols, the Mouse HGF CLIA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of HGF in biological processes and disease pathways. Order your kit today and unlock new insights into HGF biology.
Product Name:
Mouse HGF (Hepatocyte Growth Factor) CLIA Kit
SKU:
MOES00358
Target:
Mouse HGF (Hepatocyte Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HGF. You can calculate the concentration of Mouse HGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
93-107
90-105
Average (%)
106
99
97
1:4
Range (%)
103-119
89-103
96-110
Average (%)
109
96
102
1:8
Range (%)
95-111
99-116
100-117
Average (%)
102
107
107
1:16
Range (%)
84-97
95-113
88-102
Average (%)
90
103
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-109
104
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.38
256.01
937.95
86.51
236.3
958.9
Standard deviation
8.11
27.98
88.45
10.75
21.13
59.36
C V (%)
8.68
10.93
9.43
12.43
8.94
6.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HGF in samples. No significant cross-reactivity or interference between Mouse HGF and analogues was observed.
