The Mouse HIF-1 Alpha (Hypoxia-Inducible Factor 1 Alpha) CLIA Kit is a cutting-edge tool designed for the precise measurement of HIF-1 Alpha levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.HIF-1 Alpha is a critical transcription factor that plays a key role in cellular response to hypoxia, regulating the expression of genes involved in oxygen homeostasis, metabolism, and angiogenesis.
Dysregulation of HIF-1 Alpha has been implicated in various diseases, including cancer, ischemic diseases, and inflammatory disorders, making it a valuable target for studying disease mechanisms and developing novel therapeutic interventions.With its advanced technology and reliable performance, the Mouse HIF-1 Alpha CLIA Kit is an indispensable tool for researchers looking to explore the role of HIF-1 Alpha in physiology and disease, paving the way for new insights and potential treatment strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HIF-1α. You can calculate the concentration of Mouse HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
100-115
100-114
Average (%)
93
108
106
1:4
Range (%)
87-100
97-113
95-111
Average (%)
93
103
102
1:8
Range (%)
97-111
100-116
100-115
Average (%)
102
106
106
1:16
Range (%)
100-114
100-118
94-108
Average (%)
108
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
106
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.91
242.22
701.72
100.14
223.7
707.8
Standard deviation
12.31
25.77
76.84
11.16
24.96
76.87
C V (%)
12.97
10.64
10.95
11.14
11.16
10.86
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HIF-1α concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Mouse HIF-1α in samples. No significant cross-reactivity or interference between Mouse HIF-1α and analogues was observed.
