The Mouse HJV (Hemojuvelin) CLIA Kit is a valuable tool for the quantitative measurement of Hemojuvelin levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional precision and accuracy, allowing for the reliable detection of HJV levels in various experimental samples.Hemojuvelin is a key regulator of iron metabolism and plays a critical role in the pathogenesis of iron-related disorders such as hemochromatosis.
By accurately measuring Hemojuvelin levels, researchers can gain valuable insights into the mechanisms underlying iron homeostasis and develop targeted therapies for iron disorders.Whether studying iron metabolism, hemochromatosis, or related conditions, the Mouse HJV CLIA Kit provides researchers with a powerful tool to investigate the role of Hemojuvelin in disease pathology and develop novel treatment strategies.
Product Name:
Mouse HJV (Hemojuvelin) CLIA Kit
SKU:
MOES00354
Target:
Mouse HJV (Hemojuvelin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HJV. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HJV and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HJV, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HJV. You can calculate the concentration of Mouse HJV in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
97-114
90-105
Average (%)
91
104
97
1:4
Range (%)
101-118
89-102
97-109
Average (%)
108
95
103
1:8
Range (%)
88-100
89-104
98-114
Average (%)
95
95
106
1:16
Range (%)
101-118
88-103
91-104
Average (%)
108
94
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
92-105
100
Cell culture media (n=5)
101-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.83
579.49
1411.13
228.24
614.56
1299.24
Standard deviation
17.41
47.63
148.59
20.61
71.6
117.45
C V (%)
8.22
8.22
10.53
9.03
11.65
9.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HJV concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HJV in samples. No significant cross-reactivity or interference between Mouse HJV and analogues was observed.
