The Mouse HNF1B (Hepatocyte Nuclear Factor 1 Beta) CLIA Kit is a powerful tool for the precise measurement of HNF1B levels in mouse samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research endeavors.HNF1B is a key transcription factor that plays a vital role in regulating gene expression in the liver and other tissues. Dysregulation of HNF1B has been linked to various metabolic disorders, including diabetes and liver diseases.
Therefore, monitoring HNF1B levels can provide valuable insights into these conditions and aid in the development of potential treatments.Whether you are studying metabolic disorders, liver diseases, or gene expression regulation, the Mouse HNF1B CLIA Kit is an indispensable tool for your research needs. Trust AssayGenie's expertise to deliver reliable and robust results for your experiments.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HNF1b. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HNF1b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HNF1b, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HNF1b. You can calculate the concentration of Mouse HNF1b in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-120
100-114
87-101
Average (%)
109
106
92
1:4
Range (%)
87-101
94-108
84-100
Average (%)
94
99
91
1:8
Range (%)
100-115
84-97
102-118
Average (%)
107
91
109
1:16
Range (%)
84-100
99-114
84-98
Average (%)
91
107
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
104
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
90-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.66
21.6
69.35
10.31
20.04
68.98
Standard deviation
0.96
1.89
4.62
1.28
2.18
5.7
C V (%)
9.01
8.75
6.66
12.42
10.88
8.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HNF1b concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HNF1b in samples. No significant cross-reactivity or interference between Mouse HNF1b and analogues was observed.
