The Mouse HS-CRP (High Sensitivity C-Reactive Protein) CLIA Kit is a reliable and accurate tool for measuring levels of CRP in mouse serum samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for research applications.CRP is an important marker of inflammation and is commonly used in the study of various diseases such as cardiovascular diseases, autoimmune disorders, and infectious diseases.
Monitoring CRP levels can provide valuable insights into the body's inflammatory response and disease progression.With easy-to-follow instructions and quick assay protocols, the Mouse HS-CRP CLIA Kit is an essential tool for researchers looking to investigate the role of CRP in disease development and progression in mouse models. Order now to streamline your research and uncover new insights into inflammatory processes.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse hs-CRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse hs-CRP. You can calculate the concentration of Mouse hs-CRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-99
92-106
89-102
Average (%)
90
99
96
1:4
Range (%)
101-116
101-115
87-100
Average (%)
109
107
93
1:8
Range (%)
93-107
97-113
89-100
Average (%)
98
104
94
1:16
Range (%)
92-108
88-100
95-111
Average (%)
99
94
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
100-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.12
139.38
366.28
47.14
132.97
400.03
Standard deviation
4.42
11.05
24.14
5.57
10.48
27.32
C V (%)
8.82
7.93
6.59
11.82
7.88
6.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse hs-CRP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse hs-CRP in samples. No significant cross-reactivity or interference between Mouse hs-CRP and analogues was observed.
