The Mouse HSF1 (Heat Shock Factor 1) CLIA Kit is a powerful tool for the quantitative detection of HSF1 levels in mouse samples. This kit offers high sensitivity and specificity, providing researchers with reliable and accurate results for their experiments.HSF1 is a key transcription factor that regulates the expression of heat shock proteins in response to cellular stress. It plays a crucial role in maintaining cellular homeostasis and protecting cells from damage.
Dysregulation of HSF1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic diseases, making it a valuable target for therapeutic interventions.With its user-friendly protocol and high-performance characteristics, the Mouse HSF1 CLIA Kit is an essential asset for researchers studying the intricate mechanisms of cellular stress response and potential therapeutic strategies.
Product Name:
Mouse HSF1 (Heat Shock Factor 1) CLIA Kit
SKU:
MOES00326
Target:
Mouse HSF1 (Heat Shock Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HSF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HSF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HSF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HSF1. You can calculate the concentration of Mouse HSF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
100-112
94-108
Average (%)
105
106
100
1:4
Range (%)
88-102
90-103
84-95
Average (%)
95
95
90
1:8
Range (%)
84-95
102-119
101-112
Average (%)
90
109
107
1:16
Range (%)
89-102
90-104
91-106
Average (%)
96
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.46
4.9
0.46
1.5
4.74
Standard deviation
0.05
0.16
0.52
0.05
0.16
0.48
C V (%)
10.0
10.96
10.61
10.87
10.67
10.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HSF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HSF1 in samples. No significant cross-reactivity or interference between Mouse HSF1 and analogues was observed.
