The Mouse HSF2 (Heat Shock Transcription Factor 2) CLIA Kit is designed for the accurate detection of HSF2 levels in mouse serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying the role of heat shock proteins in cellular stress responses.HSF2 is a key transcription factor that regulates the expression of heat shock proteins in response to stress conditions such as heat shock, oxidative stress, and inflammation.
It plays a critical role in maintaining cellular homeostasis and protecting cells from damage, making it a valuable target for investigating various diseases and developing therapeutic strategies.Whether studying the molecular mechanisms of stress response pathways or exploring potential therapeutic interventions, the Mouse HSF2 CLIA Kit provides researchers with a powerful tool for advancing their understanding of cellular stress and its impact on health and disease.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HSF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HSF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HSF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HSF2. You can calculate the concentration of Mouse HSF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
96-109
96-114
Average (%)
101
102
104
1:4
Range (%)
88-101
103-116
87-99
Average (%)
95
109
93
1:8
Range (%)
88-103
98-112
94-106
Average (%)
95
106
100
1:16
Range (%)
92-108
85-98
88-101
Average (%)
99
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-98
90
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.93
22.74
70.14
9.93
23.79
64.21
Standard deviation
1.24
1.86
8.02
1.17
2.66
3.88
C V (%)
11.34
8.18
11.43
11.78
11.18
6.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HSF2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HSF2 in samples. No significant cross-reactivity or interference between Mouse HSF2 and analogues was observed.
